| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 115 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-ACK1 (Tyr284) Rabbit Polyclonal Antibody detects endogenous levels of ACK1 only when phosphorylated at Tyr284. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
ACK1 also known as Activated Cdc42-Associated Kinase 1, TNK2 is a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. ACK1 plays a crucial role in cellular signaling pathways by acting as a tyrosine kinase that specifically interacts with the GTP-bound form of Cdc42Hs, a member of the Ras-related Rho subfamily of GTP-binding proteins. This interaction inhibits the GTPase activity of Cdc42Hs, thereby promoting its active state and influencing cytoskeletal organization and cellular morphology. A unique sequence of 47 amino acids located C-terminal to the SH3 domain of ACK1 is essential for this binding, highlighting ACK1′s structural significance in maintaining the active form of Rho family GTPases. |
| Product Image Gallery | |
 Western blot analysis of extracts on HepG2 cells treated with EGF, using Phospho-ACK1 (Tyr284) Rabbit Polyclonal Antibody (EAB10613) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
Predicted band size: 115 kDa; Observed band size: 120 kDa  Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue, using Phospho-ACK1 (Tyr284) Rabbit Polyclonal Antibody (EAB10613) at 1:100 dilution.
 Immunofluorescence analysis of A549 cells, using Phospho-ACK1 (Tyr284) Rabbit Polyclonal Antibody (EAB10613) at 1:100 dilution.
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