| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 174 | Human, Monkey |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Topoisomerase IIα Rabbit Polyclonal Antibody detects endogenous levels of Topoisomerase IIα protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Topoisomerase IIα also known as Topo IIα is a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. Topoisomerase IIα plays a critical role in regulating the topological state of DNA, facilitating vital cellular processes including DNA replication, transcription, and chromosome segregation. By introducing transient double-strand breaks and subsequently rejoining DNA strands, Topoisomerase IIα alleviates DNA supercoiling and tangling, thereby maintaining genomic stability and ensuring proper cell division. The functionality of Topoisomerase IIα is particularly important in cell proliferation and has been identified as a key target for numerous anticancer drugs, underscoring its significance in cancer research and therapy. |
| Product Image Gallery | |
 Western blot analysis of extracts on HT29, COS7, Jurkat cells, using Topoisomerase IIα Rabbit Polyclonal Antibody (EAB10586) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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