| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IP, IHC-P, IF, ELISA | N/A | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IP=1:20-200 IHC-P=1:50-300 IF=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Ubiquitin Rabbit Polyclonal Antibody detects endogenous levels of Ubiquitin protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Ubiquitin is a highly conserved small protein composed of 76 amino acids, characterized by a compact, beta-grasp fold that is crucial for interacting with cellular proteins and enzymes. This structural integrity allows Ubiquitin to form covalent isopeptide bonds with lysine residues on target proteins, facilitating polyubiquitin chain formation essential for signaling pathways related to protein degradation, DNA repair, and cell cycle regulation. The ability of Ubiquitin to adopt different conformations enables tagging abnormal, foreign, and improperly folded proteins for degradation by the 26S Proteasome, maintaining cellular homeostasis and protein quality control. Additionally, Ubiquitin′s structural features enable participation in signal transduction cascade regulation by modulating stability and activity of key regulatory proteins such as IκB-α and p27. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, HEK293 cells, using Ubiquitin Rabbit Polyclonal Antibody (EAB10564) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human liver tissue, using Ubiquitin Rabbit Polyclonal Antibody (EAB10564) at 1:100 dilution.
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