| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC | 42 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:10000-50000 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Alpha Smooth Muscle Actin Mouse Monoclonal Antibody detects endogenous levels of Alpha Smooth Muscle Actin protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Actins are highly conserved proteins involved in cell motility, structure and integrity. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility. Smooth Muscle Actin is predominantly located in the cytoplasm, where dynamic filaments form that are essential for various cellular processes, including motility, division, and intracellular transport. Smooth Muscle Actin′s ability to polymerize and depolymerize allows cells to adapt their shape and movement in response to external stimuli, which is particularly important in smooth muscle contraction and relaxation. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, NIH/3T3, Rat Brain cells, using Alpha Smooth Muscle Actin Mouse Monoclonal Antibody (EAB10225) at 1:50000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human testis tissue, using Alpha Smooth Muscle Actin Mouse Monoclonal Antibody (EAB10225) at 1:200 dilution(4° overnight).
 Immunohistochemical analysis of paraffin-embedded Mouse cecal tissue, using Alpha Smooth Muscle Actin Mouse Monoclonal Antibody (EAB10225) at 1:500 dilution(4° overnight).
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