| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 130 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | COL17A1 Rabbit Polyclonal Antibody detects endogenous levels of COL17A1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
COL17A1 also known as Collagen type XVII alpha, BP180 is a collagen family hemidesmosome anchorage molecule of basal keratinocytes that promotes stable epidermal-dermal adhesion and wound healing. COL17A1 is critical for maintaining keratocyte and hair follicle stem cells, promoting cell motility and increased self-renewing capability. The protein functions by organizing actin and keratin filament networks via its interaction with intracellular scaffold protein plectin (PLEC) and integrin β4 in the hemidesmosomes. High expression of COL17A1 is associated with cervical, colorectal, pancreatic, and lung cancers. It induces a tumor-favorable microenvironment and promotes invasion and metastasis. |
| Product Image Gallery | |
 Western blot analysis of extracts on NIH/3T3, HepG2 cells, using COL17A1 Rabbit Polyclonal Antibody (EAB13066) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue, using COL17A1 Rabbit Polyclonal Antibody (EAB13066) at 1:100 dilution.
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