| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 28 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-14-3-3 theta (Ser232) Rabbit Polyclonal Antibody detects endogenous levels of 14-3-3 theta only when phosphorylated at Ser232. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
14-3-3 theta is a member of the 14-3-3 protein family, which plays a crucial role in regulating various cellular processes, particularly in cancer biology, where 14-3-3 theta protein is involved in apoptosis, mitogenic signaling, and cell-cycle checkpoints. 14-3-3 theta protein is primarily located in the cytoplasm, where 14-3-3 theta protein interacts with a wide range of partner proteins, facilitating proper function and localization. Cytoplasmic presence of 14-3-3 theta protein allows regulation of signaling pathways essential for cell survival and proliferation. Through sequestering target proteins in the cytoplasm, 14-3-3 theta protein prevents degradation and modulates activity, influencing critical cellular responses to stress and growth signals. Formation of dimers by 14-3-3 proteins creates binding sites for ligands sharing consensus binding motifs, underscoring importance in maintaining cellular homeostasis. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela cells, using Phospho-14-3-3 theta (Ser232) Rabbit Polyclonal Antibody (EAB10511) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
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