| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 28 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | 14-3-3 epsilon Rabbit Polyclonal Antibody detects endogenous levels of 14-3-3 epsilon protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
14-3-3 epsilon is a member of the 14-3-3 protein family, which plays a crucial role in regulating cellular processes such as apoptosis, mitogenic signaling, and cell-cycle checkpoints, making 14-3-3 epsilon significant in cancer biology. 14-3-3 epsilon protein is primarily located in the cytoplasm, where 14-3-3 epsilon interacts with a variety of target proteins, facilitating proper function and localization. Cytoplasmic presence of 14-3-3 proteins is essential for sequestering and regulating ligands, which often contain a 14-3-3 consensus binding motif and are typically phosphorylated on serine or threonine residues. By binding to these phosphorylated proteins, 14-3-3 epsilon can modulate activity, stability, and interactions, thereby influencing critical signaling pathways. |
| Product Image Gallery | |
 Western blot analysis of extracts on HepG2, Jurkat cells, using 14-3-3 epsilon Rabbit Polyclonal Antibody (EAB10504) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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