| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IP, IHC-P, IF/ICC, ELISA | 32 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IP=1:20-200 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Atg5 Mouse Monoclonal Antibody detects endogenous levels of Atg5 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Atg5 also known as APG5 is a crucial component of the autophagy machinery, a fundamental cellular process responsible for degradation and recycling of cytoplasmic components to maintain cellular homeostasis, especially under stress conditions like nutrient starvation. Located primarily in the cytoplasm, Atg5 translocates to the forming autophagosome membrane where Atg5 forms a complex with Atg12 and Atg16L1, facilitating elongation and closure of autophagosomes. This localization is essential for proper formation of autophagosomes, which sequester damaged organelles and proteins for degradation, thereby preventing accumulation of cellular debris and promoting cell survival. Atg5 plays a role in regulation of apoptosis, linking autophagy to programmed cell death pathways and influencing cell fate decisions. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, 293T, SH-SY5Y cells, using Atg5 Mouse Monoclonal Antibody (EAB10495) at 1:2000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 32 kDa; Observed band size: 55 kDa  Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue, using Atg5 Mouse Monoclonal Antibody (EAB10495) at 1:200 dilution.
 Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue, using Atg5 Mouse Monoclonal Antibody (EAB10495) at 1:200 dilution.
|
