| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 45 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Atg4A Rabbit Polyclonal Antibody detects endogenous levels of Atg4A protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Atg4A also known as APG4A is a crucial cysteine protease consisting of 398 amino acids that plays a vital role in autophagy, essential for cellular homeostasis and degradation of damaged organelles and proteins. Located primarily in the cytoplasm, Atg4A is a protein responsible for cleaving the C-terminal part of specific proteins, including GABARAP and MAP1LC3, facilitating autophagosome formation - double-membrane vesicles that engulf cellular debris for lysosomal degradation. Proper functioning of Atg4A regulates autophagy and influences cellular responses to stress and nutrient deprivation. Multiple alternatively spliced isoforms of Atg4A exist, and N-ethylmaleimide can inhibit Atg4A activity, highlighting post-translational modifications in regulatory mechanisms. |
| Product Image Gallery | |
 Western blot analysis of extracts on COLO205, Raw264.7 cells, using Atg4A Rabbit Polyclonal Antibody (EAB10491) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemistry analysis of paraffin-embedded Human colon cancer tissue, using Atg4A Rabbit Polyclonal Antibody (EAB10491) at 1:100 dilution.
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