| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IP, IHC-P, IF/ICC | 68 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IP=1:100-500 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Lamin B1 Mouse Monoclonal Antibody detects endogenous levels of Lamin B1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Lamin B1 also known as LMNB1 is one of the two B-type lamin proteins and is a component of the nuclear lamina. Lamin B1 plays a crucial role in maintaining the structural integrity of the nuclear envelope and is a key component of the nuclear lamina, which provides mechanical support to the nucleus and helps organize chromatin. Proper localization of Lamin B1 within the nucleus is essential for regulating gene expression and ensuring cellular stability, as disruptions in its function can lead to various diseases, including laminopathies and cancer. Additionally, Lamin B1 undergoes post-translational modifications such as farnesylation and phosphorylation, which are vital for its function and stability; increased phosphorylation of Lamin B1 is associated with the disintegration of the nuclear envelope during apoptosis, highlighting its importance in cellular processes. |
| Product Image Gallery | |
 Western blot analysis of extracts on HepG2, 293T, Mouse Brain, Rat Brain cells, using Lamin B1 Mouse Monoclonal Antibody (EAB10439) at 1:5000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
 Lane1: Input: Mouse Brain Tissue
Lane2: IP product: IP dilute 1:200 |
