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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 82 | Human, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-eEF2K (Ser366) Rabbit Polyclonal Antibody detects endogenous levels of eEF2K only when phosphorylated at Ser366. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
eEF2K is predominantly located in the cytoplasm, where eEF2K interacts with calcium and calmodulin, which are essential for activation. Notably, eEF2K undergoes post-translational modifications, including phosphorylation at specific serine residues, which further influence activity and stability. For instance, phosphorylation at Ser 359 and Ser 366, induced by factors such as insulin-like growth factor-1 and p70 S6 kinase, respectively, leads to inactivation of eEF2K, particularly under low calcium conditions. This intricate regulation underscores the importance of eEF2K in maintaining cellular homeostasis and responding to environmental changes. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela cells treated with Serum, using Phospho-eEF2K (Ser366) Rabbit Polyclonal Antibody (EAB10413) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
Predicted band size: 82 kDa; Observed band size: 105 kDa |
