| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC | 21 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Bax Mouse Monoclonal Antibody detects endogenous levels of Bax protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Bax belongs to the BCL2 protein family. Bax is primarily located in the cytosol and translocates to the mitochondria during the early stages of apoptosis, where Bax facilitates the release of cytochrome c, a key event that triggers the apoptotic cascade. Understanding the localization and function of Bax is vital, as Bax activity is tightly regulated by interactions with anti-apoptotic proteins like Bcl-2, which can inhibit pro-apoptotic effects. The balance between Bax and Bcl-2 is critical for maintaining cellular homeostasis, and dysregulation of this balance can lead to various diseases, including cancer. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, PC12, C2C12 cells, using Bax Mouse Monoclonal Antibody (EAB10275) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue, using Bax Mouse Monoclonal Antibody (EAB10275) at 1:200 dilution.
 Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue, using Bax Mouse Monoclonal Antibody (EAB10275) at 1:200 dilution.
 Immunohistochemical analysis of paraffin-embedded Rat testis tissue, using Bax Mouse Monoclonal Antibody (EAB10275) at 1:200 dilution.
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