| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 88 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Cyclin F Rabbit Polyclonal Antibody detects endogenous levels of Cyclin F protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Cyclin F also known as FBX1 is a member of the cyclin family. Cyclin F plays a crucial role in cell cycle regulation, being the largest member of the cyclin family, and contains a unique PEST-rich C-terminus along with a cyclin box region closely related to cyclins A and B. Cyclin F′s function is particularly important in controlling the G2 to M transition, a critical phase in cell division, where proper regulation ensures accurate DNA replication and distribution to daughter cells. Cyclin F expression occurs throughout the cell cycle but shows dynamic fluctuations, peaking during the G2 phase, which is essential for preparing cells for mitosis. Cyclin F′s regulated subcellular localization, primarily in the nucleus with notable perinuclear staining in some cells, underscores its significance in cellular processes and highlights its potential as a target for studying cell cycle-related disorders. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, HuvEc cells, using Cyclin F Rabbit Polyclonal Antibody (EAB13085) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemistry analysis of paraffin-embedded Human colorectal cancer tissue, using Cyclin F Rabbit Polyclonal Antibody (EAB13085) at 1:100 dilution.
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