| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 47 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Cyclin E2 Rabbit Polyclonal Antibody detects endogenous levels of Cyclin E2 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Cyclin E2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclin E2 plays a crucial role in cell cycle regulation, particularly during the G1/S phase transition, where cyclin E2 interacts with cyclin-dependent kinases Cdk2 and Cdk3, as well as cyclin-dependent kinase inhibitors p27 and p21. This interaction is vital for proper cell cycle progression, as cyclin E2 helps regulate Cdk2 activity, which is essential for transitioning from G1 phase to S phase, where DNA replication occurs. Notably, cyclin E2 expression remains low in non-transformed cells but shows significant elevation in tumor-derived cells, highlighting cyclin E2′s potential as a cancer progression biomarker. |
| Product Image Gallery | |
 Western blot analysis of extracts on COLO205, Jurkat cells, using Cyclin E2 Rabbit Polyclonal Antibody (EAB12124) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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