| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC | 29 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:2000-10000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | PCNA Mouse Monoclonal Antibody detects endogenous levels of PCNA protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PCNA(Proliferating cell nuclear antigen) is a critical nuclear protein involved in DNA replication and repair in eukaryotic cells. PCNA forms a ring structure that acts as a scaffold for recruiting proteins, supporting DNA polymerases during replication. PCNA plays key roles in DNA metabolism, chromatin remodeling, cell cycle regulation, and DNA repair pathways, ensuring high-fidelity DNA replication. PCNA is regulated by post-translational modifications, allowing dynamic response to DNA damage and maintenance of genomic integrity. Dysregulation of PCNA is linked to cancer, making PCNA an important biomarker for prognosis and a potential therapeutic target. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, 293T, NIH/3T3, Rat brain cells, using PCNA Mouse Monoclonal Antibody (EAB10347) at 1:5000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
 Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue, using PCNA Mouse Monoclonal Antibody (EAB10347) at 1:200 dilution.
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