| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 55 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-MDM2 (Ser166) Rabbit Polyclonal Antibody detects endogenous levels of MDM2 protein only when phosphorylated at Ser166. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
MDM2 also known as murine double minute 2 is an E3 ubiquitin-protein ligase. MDM2 plays a critical role in regulating the tumor suppressor protein p53 by targeting p53 for ubiquitination and proteasomal degradation. Nuclear localization of MDM2 is essential for interaction with p53, as MDM2 binds directly to p53′s transactivation domain, inhibiting transcriptional activity and promoting degradation. This regulation is crucial because p53 controls cell cycle progression and induces cell cycle arrest or apoptosis in response to DNA damage. Overexpression or amplification of MDM2 can lead to decreased p53 activity, allowing cells with damaged DNA to proliferate unchecked, which contributes to tumorigenesis. MDM2 is frequently overexpressed in various human cancers, making MDM2 an important focus in cancer research and a potential target for therapeutic intervention. |
| Product Image Gallery | |
 Western blot analysis of extracts on MCF7 cells, using Phospho-MDM2 (Ser166) Rabbit Polyclonal Antibody (EAB10166) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
Predicted band size: 55 kDa; Observed band size: 55,90 kDa  Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue, using Phospho-MDM2 (Ser166) Rabbit Polyclonal Antibody (EAB10166) at 1:100 dilution.
 Immunofluorescence analysis of A549 cell, using Phospho-MDM2 (Ser166) Rabbit Polyclonal Antibody (EAB10166) at 1:200 dilution.
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