| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 52 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Parkin Rabbit Polyclonal Antibody detects endogenous levels of Parkin protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Parkin also known as PRKN is a component of a multiprotein E3 ubiquitin ligase complex that mediates the targeting of substrate proteins for proteasomal degradation. Parkin plays a pivotal role in tagging proteins for degradation, a process essential for maintaining cellular homeostasis. Structurally, Parkin contains an N-terminal ubiquitin-like domain and several zinc-finger motifs, including RING (Really Interesting New Gene) domains, which are critical for its E3 ligase activity. These domains facilitate interactions with substrate proteins and the ubiquitin-conjugating machinery, enabling Parkin to regulate protein turnover effectively. The integrity of these structural motifs is vital for Parkin′s function, as mutations within these regions often disrupt its activity and are linked to autosomal recessive juvenile Parkinson′s disease. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Jurkat, HuvEc cells, using Parkin Rabbit Polyclonal Antibody (EAB10305) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue, using Parkin Rabbit Polyclonal Antibody (EAB10305) at 1:100 dilution.
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