| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IF/ICC, ELISA | 133 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-eNOS/NOS3 (Ser1177) Rabbit Polyclonal Antibody detects endogenous levels of eNOS/NOS3 only when phosphorylated at Ser1177. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
NOS3 also known as eNOS plays a crucial role in the cardiovascular system by synthesizing nitric oxide (NO), a signaling molecule that regulates vascular tone and blood flow. Proper functioning of NOS3 is vital for maintaining endothelial health and preventing cardiovascular diseases, as NOS3 helps mediate vasodilation and inhibit platelet aggregation. NOS3 is primarily located in endothelial cells lining blood vessels, where NOS3 activity is tightly regulated by calcium levels and calmodulin binding, ensuring NO production is responsive to physiological demands. Dysregulation of NOS3 can lead to various pathologies, including hypertension and atherosclerosis, highlighting NOS3′s importance in vascular biology and potential as a therapeutic target. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela cells treated with Insulin, using Phospho-eNOS/NOS3 (Ser1177) Rabbit Polyclonal Antibody (EAB10274) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
Predicted band size: 133 kDa; Observed band size: 130-140 kDa |
