| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 161 | Human, Mouse, Rat, Monkey |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-nNOS/NOS1 (Ser852) Rabbit Polyclonal Antibody detects endogenous levels of nNOS/NOS1 only when phosphorylated at Ser852. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
nNOS also known as NOS1 belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. nNOS is predominantly localized in neurons, where NOS1 interacts with key proteins such as PSD-95, which anchors nNOS to the postsynaptic density, and calmodulin, which regulates enzymatic activity. These interactions are crucial for the precise spatial and temporal production of NO, facilitating NO′s role in modulating synaptic strength and plasticity. Understanding the protein-protein interactions of nNOS is vital for elucidating the mechanisms underlying neuronal signaling and the pathophysiology of neurological disorders where NO signaling may be disrupted. |
| Product Image Gallery | |
 Western blot analysis of extracts on A549 cells, using Phospho-nNOS/NOS1 (Ser852) Rabbit Polyclonal Antibody (EAB10271) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
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