| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 137 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | HER2/ErbB2 Rabbit Polyclonal Antibody detects endogenous levels of HER2/ErbB2 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
ErbB2 also known as HER2, CD340, Neu is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases, which consists of receptor tyrosine kinases that play critical roles in cell signaling pathways related to growth and differentiation. ErbB2 is primarily located on the cell membrane, where binding with ligands can form homodimers or heterodimers with other EGFR family members, such as ErbB3. This dimerization activates downstream signaling cascades, including the phosphoinositide 3-kinase pathway, which regulates cell proliferation, survival, and motility. Aberrant expression of ErbB2 is associated with various cancers, particularly breast carcinoma, where amplification or overexpression occurs in 20-30% of cases, making ErbB2 a significant biomarker for disease prognosis and a potential target for therapeutic interventions. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, AD293 cells, using HER2/ErbB2 Rabbit Polyclonal Antibody (EAB10248) at 1:2000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 137 kDa; Observed band size: 180 kDa  Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue, using HER2/ErbB2 Rabbit Polyclonal Antibody (EAB10248) at 1:100 dilution.
 Immunofluorescence analysis of Hela cell, using HER2/ErbB2 Rabbit Polyclonal Antibody (EAB10248) at 1:100 dilution.
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