| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 53 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-IkBε (Ser22) Rabbit Polyclonal Antibody detects endogenous levels of IkBε protein only when phosphorylated at Ser22. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
IkBε plays a crucial role in the regulation of the NF-kB signaling pathway, which is essential for controlling immune responses, cell proliferation, and survival. IkBε is primarily located in the cytoplasm, where IkBε binds to NF-kB dimers, preventing their translocation to the nucleus and thereby inhibiting the expression of target genes involved in inflammation and immune responses. Proper functioning of IkBε is vital, as dysregulation can lead to various diseases, including cancer and autoimmune disorders. |
| Product Image Gallery | |
 Western blot analysis of extracts on Jurkat cells treated with TNFalpha, using Phospho-IkBε (Ser22) Rabbit Polyclonal Antibody (EAB13137) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
Predicted band size: 53 kDa; Observed band size: 38 kDa |
