| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, ELISA | 19 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | CHOP Mouse Monoclonal Antibody detects endogenous levels of CHOP protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
CHOP also known as GADD153, DDIT3 is a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. CHOP is essential for dimerization with other C/EBP family members and for binding to specific DNA sequences to regulate gene expression. The structural integrity of the bZIP domain determines CHOP′s ability to form homodimers or heterodimers, thereby influencing regulatory functions in response to cellular stressors disturbances. This structural feature allows CHOP to undergo conformational changes that enable interaction with nonclassical DNA binding sites, effectively altering the transcriptional landscape to induce cell cycle arrest at the G1 to S phase transition and promote apoptotic pathways. |
| Product Image Gallery | |
 Western blot analysis of extracts on A375, Mouse liver cells, using CHOP Mouse Monoclonal Antibody (EAB10179) at 1:2000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 19 kDa; Observed band size: 19-30 kDa  mmunohistochemical analysis of paraffin-embedded Human pancreas carcinoma tissue, using CHOP Mouse Monoclonal Antibody (EAB10179) at 1:200 dilution.
 Immunohistochemical analysis of paraffin-embedded Human stomach carcinoma tissue, using CHOP Mouse Monoclonal Antibody (EAB10179) at 1:200 dilution.
|
