| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 36 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-C/EBPβ (Thr235) Rabbit Polyclonal Antibody detects endogenous levels of C/EBPβ only when phosphorylated at Thr235. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
C/EBPβ also known as CEBPB is a crucial transcription factor belonging to the CCAAT-enhancer binding protein family, playing a significant role in the differentiation of various cell types, including liver, adipose tissue, blood cells, and the endocrine pancreas. C/EBP β is particularly important due to its involvement in the regulation of the acute phase response in hepatocytes, a critical process for the body′s response to inflammation and injury. C/EBP β exists in multiple isoforms, generated through alternative translational initiation at several AUG start sites, which allows for a diverse range of functional activities. The phosphorylation of C/EBP β is essential for DNA binding capability, and interaction with Stat3 is vital for the interleukin 6-mediated transcription of the C/EBP β gene, linking C/EBP β directly to the acute phase response in liver cells. |
| Product Image Gallery | |
 Western blot analysis of lysates from HepG2 cells treated with EGF, using Phospho-C/EBPβ (Thr235) Rabbit Polyclonal Antibody (EAB10175) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
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