| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 84, 54 | Human, Mouse, Rat, Monkey, Pig |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-PI3 Kinase p85 (Tyr467)/p55 (Tyr199) Rabbit Polyclonal Antibody detects endogenous levels of p85/p55 protein only when phosphorylated at Tyr467/Tyr199. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PI3 kinase also known as phosphoinositide-3-kinase is a heterodimer composed of a regulatory subunit (p85) and a catalytic sub unit (p110), and phosphorylates phosphoinositides at the 3′ OH position of their inositol rings. The p85 subunits, including p85α, p85β, and p55γ, serve as regulatory adapters that anchor the catalytic subunit to activated receptor tyrosine kinases at the plasma membrane. Such localization is essential for signal transduction, as positioning near lipid substrates initiates pathways like AKT/mTOR that regulate cell growth, survival, and metabolism. PI 3-kinase p85α (also known as GRB1 or phosphatidylinositol 3-kinase regulatory 1) plays a key role in insulin signaling, and dysregulation has been linked to conditions such as insulin resistance and cancer. Post-translational modifications, including tyrosine phosphorylation and ubiquitination, modulate p85 subunit activity and stability, further refining signaling pathway control. |
| Product Image Gallery | |
 Western blot analysis of extracts on COS7 cells treated with H2O2, using Phospho-PI3 Kinase p85 (Tyr467)/p55 (Tyr199) Rabbit Polyclonal Antibody (EAB10111) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
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