| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 84 | Human, Mouse, Rat, Pig |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | PI3 Kinase p85α Rabbit Polyclonal Antibody detects endogenous levels of PI3 Kinase p85α protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PI3 kinase p85α also known as p85alpha, PIK3R1 is a critical component of phosphatidylinositol 3-kinase (PI3K), which regulates key cellular processes such as growth, survival, metabolism, and motility. The p85α subunit serves as a regulatory adapter that couples the catalytic p110 subunit to activated protein tyrosine kinases, controlling the kinase′s activity and localization. PI3K′s activity is central to intracellular signaling pathways, including the AKT/mTOR pathway, which is vital for cell proliferation and survival. The p85α subunit contains SH2 and SH3 domains that mediate interactions with phosphorylated tyrosine residues on receptor tyrosine kinases and other signaling proteins, ensuring precise regulation of PI3K signaling. Dysregulation of PI3K activity, often through mutations or altered expression of p85α, is implicated in various cancers and metabolic disorders. |
| Product Image Gallery | |
 Western blot analysis of extracts on HepG2, HuvEc, Raw264.7 cells, using PI3 Kinase p85α Rabbit Polyclonal Antibody (EAB13198) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human breast caricnoma tissue, using PI3 Kinase p85α Rabbit Polyclonal Antibody (EAB13198) at 1:200 dilution.
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