| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IF/ICC, ELISA | 48, 52 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-Smad2/Smad3 (Thr8) Rabbit Polyclonal Antibody detects endogenous levels of Smad2/Smad3 only when phosphorylated at Thr8. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Smad2 and Smad3 also known as MAD2 and MAD3 respectively, are essential components of the Transforming Growth Factor-beta (TGFβ) signaling pathway. These proteins interact directly with Smad4 (DPC4), forming complexes that translocate to the nucleus to regulate gene transcription involved in cell proliferation, differentiation, and apoptosis. Additionally, Smad2/3 interact with various transcription factors and co-regulators, such as FOXH1 and p300, to modulate the expression of target genes. The interaction between Smad2/3 and TGFβ receptors is crucial for the phosphorylation and activation of these Smad proteins, facilitating their role in cellular homeostasis and development. |
| Product Image Gallery | |
 Western blot analysis of extracts on HepG2 cell treated with Forskolin, using Phospho-Smad2/Smad3 (Thr8) Rabbit Polyclonal Antibody (EAB10061) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
Predicted band size: 48,52 kDa; Observed band size: 48,60 kDa |
