Protein Inhibitors - Nicotinamide (CAS: 98-92-0)

Nicotinamide (CAS: 98-92-0)

Catalog #：EBC51143

Biological Activity

Synonyms

Niacinamide, Nicotinic acid amide, Vitamin B3

Chemical Name

pyridine-3-carboxamide

Application

Nicotinamide is an NAD+ and NADP+ coenzyme precursor

CAS Number

98-92-0

Purity

≥98.0%

Molecular Weight

122.1

Molecular Formula

C₆H₆N₂O

SMILES

C1=CC(=CN=C1)C(=O)N

Target & IC50

Anandamide amidohydrolase: IC50 = 3.3 μM
NAD-dependent deacetylase sirtuin 2: IC50 = 11 μM

Shipping

Gel Pack

Storage

Store at -20°C

Preparing Stock Solutions

Molecular Structure

ConcentrationSolventMass	1 mg	5 mg	10 mg
1 mM	8.1887 ml	40.9433 ml	81.8867 ml
5 mM	1.6377 ml	8.1887 ml	16.3773 ml
10 mM	0.8189 ml	4.0943 ml	8.1887 ml
50 mM	0.1638 ml	0.8189 ml	1.6377 ml

Solubility
DMSO: 22 mg/mL (180.2 mM) Ethanol: 22 mg/mL (180.2 mM) Water: 22 mg/mL (180.2 mM)

Product Description

Nicotinamide is an inhibitor of poly(ADP-ribose) polymerase (PARP-1) enzymes. Poly(ADP-ribose) polymerase-1 (PARP-1) is found as a DNA repair enzyme. The excessive activation of PARP-1, such as ischemia and trauma, can deplete cellular nicotinamide adenine dinucleotide as a substrate and leads to brain cell death eventually.

In vitro: Previous findings suggested that nicotinamide had a protective effect against PARP-1-induced astrocyte death. The transporter-mediated uptake of nicotinamide, which was extracellular pH-sensitive and common to N-methylnicotinamide, was found to be critical for prevention of PARP-1-triggered cell death.

In vivo: Type 2 diabetes was induced in Wistar rats by streptozotocin followed by nicotinamide. Test compounds and standard treatment were continued for 15 days. Results showed that there was significant normalisation of liver antioxidant enzymes compared to diabetic rats, suggesting all the tested compounds were beneficial in reducing oxidative stress.