Protein Inhibitors - ID-8 (CAS: 147591-46-6)

ID-8 (CAS: 147591-46-6)

Catalog #：EBC51109

Biological Activity

Synonyms

HY-15838, ID8, DYRK inhibitor

Chemical Name

1-(4-methoxyphenyl)-2-methyl-3-nitroindol-6-ol

Application

ID-8 is an inhibitor of DYRK

CAS Number

147591-46-6

Purity

≥98.0%

Molecular Weight

298.29

Molecular Formula

C₁₆H₁₄N₂O₄

SMILES

OC1=CC2=C(C=C1)C([N+]([O-])=O)=C(C)N2C3=CC=C(OC)C=C3

Target & IC50

DYRK

Shipping

Gel Pack

Storage

Store at -20°C

Preparing Stock Solutions

Molecular Structure

ConcentrationSolventMass	1 mg	5 mg	10 mg
1 mM	3.3524 ml	16.7622 ml	33.5244 ml
5 mM	0.6705 ml	3.3524 ml	6.7049 ml
10 mM	0.3352 ml	1.6762 ml	3.3524 ml
50 mM	0.0670 ml	0.3352 ml	0.6705 ml

Solubility
DMSO: 29.8 mg/mL (100 mM)

Product Description

ID-8 is a small molecule DYRK inhibitor. In HES2 cells, ID-8 (0.5 μM) increased hESC survival by 1.1%. The combination of ID-8 and Wnt3a increased survival by 1.7% and completely inhibited Wnt-induced morphological differentiation. Also, ID-8 significantly reduced the expression of differentiation marker gene GATA6, GSC, SOX17 and CDX2 induced by Wnt. In hESCs, ID-8 increased Wnt-mediated hESC proliferation and survival via inhibition of DYRKs. Also, DYRK family is direct targets of ID-8. In embryonic stem cells (ESCs), ID-8 (10 μM) stimulated proliferation in culture without MEFs, serum or LIF. The ID-8 induced ESCs showed the ability to form all three germ layer-derived tissues, such as liver, neurons and muscles. Also, ID-8 increased ESC differentiation and the levels of Nanog, Sox2 and Rex-1 . In mouse ESCs cultured in serum-free medium, ID-8 maintained their self-renewal and pluripotency.