Protein Inhibitors - IBMX (CAS: 28822-58-4)

IBMX (CAS: 28822-58-4)

Catalog #：EBC51868

Biological Activity

Synonyms

Isobutylmethylxanthine, 1-Methyl-3-Isobutylxanthine

Chemical Name

1-Methyl-3-(2-methylpropyl)-7H-purine-2,6-dione

Application

IBMX is a potent and nonspecific inhibitor of PDE (phosphodiesterases) and can increase intracellular cyclic AMP levels

CAS Number

28822-58-4

Purity

≥99.0%

Molecular Weight

222.24

Molecular Formula

C₁₀H₁₄N₄O₂

SMILES

O=C(N1C)N(CC(C)C)C2=C(N=CN2)C1=O

Target & IC50

PDE3: IC50 = 18 μM
PDE4: IC50 = 13 μM
PDE1: IC50 = 19 μM
PDE5 : IC50 = 32 μM

Shipping

Room Temperature

Storage

Room Temperature

Preparing Stock Solutions

Molecular Structure

ConcentrationSolventMass	1 mg	5 mg	10 mg
1 mM	4.4996 ml	22.4982 ml	44.9964 ml
5 mM	0.8999 ml	4.4996 ml	8.9993 ml
10 mM	0.4500 ml	2.2498 ml	4.4996 ml
50 mM	0.0900 ml	0.4500 ml	0.8999 ml

Solubility
DMSO: 44 mg/mL (197.98 mM) Ethanol: 7 mg/mL (31.50 mM)

Product Description

IBMX is one of the most potent and nonspecific inhibitors of cyclic nucleotide PDE (phosphodiesterases) with documented IC50 values for PDE1, 2, 3, 4, and 5 equaling 19, 50, 18, 13 and 32. IBMX is documented to raise intracellular cyclic AMP levels. In rat sensory neurons the addition of IBMX caused the release of Ca2+ from caffeine ryanodine sensitive internal stores increasing the internal Ca2+ levels and inducing a biphasic membrane current response. Through PDE inhibition IBMX has also inhibited TNFα. IBMX in breast cancer cells has also induced p21 and p27 through a PKA independent pathway. Although a nonspecific PDE inhibitor IBMX does not show inhibition of PDE8B.